Improved conditions for in situ RNA/DNA hybridization
نویسندگان
چکیده
منابع مشابه
Inversion of in situ synthesized oligonucleotides: improved reagents for hybridization and primer extension in DNA microarrays.
Oligonucleotides synthesized in array format suffer from contamination by truncated species. We have developed a method to invert DNA molecules in situ after completed synthesis. Reactive functions at the 5'-ends of the oligonucleotides are permitted to react with functions on the support before the 3'-ends are released, in effect reversing the orientation of full-length oligonucleotides, while...
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We have modified a nonradioactive (color) in situ hybridization method for the detection of mRNA populations, transcribed from the calmodulin (CaM) I gene, that exist in low abundances in tissues such as the white matter of the rat spinal cord. Our results indicate that increasing the pH of the hybridization solution from neutral to slightly alkaline (pH 8.08.5) drastically improves the detecta...
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In situ hybridization analysis of tissue mRNA concentrations remains to be accepted as a quantitative technique, even though exposure of tissue sections to photographic emulsion is equivalent to Northern blot analysis. Because of the biological importance of in situ quantification of RNA sequences within a morphological context, we evaluated the quantitative aspects of this technique. In calibr...
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ژورنال
عنوان ژورنال: FEBS Letters
سال: 1973
ISSN: 0014-5793
DOI: 10.1016/0014-5793(73)80078-x